development of a high-resolution melting method for screening r188h polymorphism in xrcc2 gene

background: the high resolution melting (hrm) method is a new scanning method for detecting unknown changes in dna and its advantages have persuaded researchers to recruit it as a screening method. objectives: here, we developed a hrm method to screen r188h snp (rs3218536) of xrcc2 and compared the results with a well known pcr-rflp technique. materials and methods: genomic dna samples from 350 healthy individuals were obtained. pcr-hrm analysis and pcr-rflp method were performed simultaneously. results: three different melting profiles corresponding to three different genotypes recognized by hrm analysis. the results of pcr-rflp showed no discrepancy. conclusions: we concluded that the hrm technique can be used as a screening method for rapid discrimination of r188h genotypes in xrcc2 gene.